Determination of Cholalic Acid and Hyodeoxycholic Acid in Qingkailing Suppository by HPLC-ELSD / 中国药房
China Pharmacy
; (12)2001.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-532023
Responsible library:
WPRO
ABSTRACT
OBJECTIVE:
To determine the contents of cholalic acid and hyodeoxycholic acid in Qingkailing suppository by HPLC-ELSD(high performance liquid chromatography with evaporative light scattering detection).METHODS:
The analytical column was Diamonsil C18(250 mm?4.6 mm, 5 ?m) and the mobile phase consisted of 0.1% formic acid-acetonitrile(gradient elution) with the temperature of the drift tube of the light scattering detector set at 110 ℃ and the flow rate of the nitrogen gas at 2.0 L?min-1.RESULTS:
The linear ranges of cholalic acid and hyodeoxycholic acid were 0.59~7.4 ?g(r=0.999 6)and 0.38~4.8 ?g(r=0.999 7),respectively,with their average recoveries at 99.20%(RSD=1.4%,n=6)and 98.57%(RSD=1.8%,n=6), respectively.CONCLUSION:
The method is simple, accurate and well-separated, and it is applicable for the quality control of Qingkailing suppository.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2001
Document type:
Article