Effects of Compound Glycyrrhizin on Chemotactic Factors' Expression in Peripheral Blood Mononuclear Cells / 中国药房
China Pharmacy
; (12)2005.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-532241
Responsible library:
WPRO
ABSTRACT
OBJECTIVE:
To study whether the therapeutic action of compound glycyrrhizin(SNMC) on allergic disorders can be achieved through inhibition on the expression of chemotactic factors in peripheral blood mononuclear cells(PBMC).METHODS:
PBMC of 15 healthy subjects were obtained and divided into 4 drug-treated groups SNMC group,dexamethasone group,mizolastine group,and loratadine group;meanwhile,a positive control group(proinflammatory stimulant was added) and a negative control group(no proinflammatory stimulant was added) were used as control.Fluorescent quantitation polymerase chain reaction was employed to determine the mRNA expression of the chemotatic factors including RANTES,MCP-1,MIP-1? and ELISA was employed to detect their concentrations in supernatant.RESULTS:
In the positive control group,both the mRNA expressions and the concentrations of the 3 chemotatic factors increased significantly,and which were significantly lower in the drug-treatment groups than in the positive control group.There were significant differences between the positive control group and dexamethasone-treated group or SNMC-treated group in the expression of MIP-1? mRNA(P
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2005
Document type:
Article