Apoptosis Expression-based Pattern Recognition System for Four Natures of Chinese Materia Medica——Comparative Study on Drug Natures of the Water Extracts of Panax ginseng,Dry Panax ginseng and Panax quinque folium against A?_(25-35)-induced Apoptosis of / 中国药房
China Pharmacy
; (12)2005.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-532772
Responsible library:
WPRO
ABSTRACT
OBJECTIVE:
To investigate the effects of water extracts of Panax ginseng(warm),dry P.ginseng(mild),P.quinque folium(cool) on the apoptosis of SH-SY5Y cells induced by ?-amyloid protein(A?25-35).METHODS:
SH-SY5Y cells were treated with A?25-35 to simulate the pathologic neuronic damage model in patients with Alzheimer's disease,and then treated with different doses of water extracts of P.ginseng(WERG),dry P.ginseng(WEG)or P.quinque folium(WEAG).The morphologic changes of the SH-SY5Y cells were observed by inverted microscope.Cell survival rate was measured by MTT assay.The percentage of apoptosis and the expression of Bax and Bcl-2 proteins were detected by Western Blotting.RESULTS:
After treatment with A?25-35 50 ?mol?L-1 for 72 hours,SH-SY5Y cells became more round and aggregated and the cell survival rate was significantly reduced(P
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2005
Document type:
Article