The ciliary beat frequency of human nasal epithelium in vitro / 中国耳鼻咽喉头颈外科

ABSTRACT

OBJECTIVE To explore the valuable information for studying human nasal mucociliary transport system by measuring the nasal ciliary beat frequency(CBF)in vitro.METHODS CBF of cultured human nasal epithelial cells was measured by highspeed digital microscopy in HBSS.RESULTS CBF of nasal epithelial cells from inferior turbinate and uncinate process of same patient has no significant difference in vitro(t=-0.087,P=0.931).CBF of normal mucosa and uncinate process from patients of sinusitis has no significant difference in vitro(t=0.419,P=0.678).CONCLUSION CBF of nasal mucosa of sinusitis can replace the CBF of normal nasal mucosa in vitro for for nasal ciliary movement studying.Because the mucosa of uncinate process is easier to obtain,it can replace the mucosa of inferior turbinate for cell culture in vitro.