A Quantitative solid-phase radioimmunoassay detecting the antibodies against group A neisseria meningococcal capsular polysaccharide / 中国免疫学杂志

ABSTRACT

A procedure of establishing Solid-phase Radioimmunoassay detecting the antibodies to group A Neisseria meningococcal capsular polysaccharide was reported in detail.The polyvinyl chloride micro-titer plate was selected as a solid-phase carrier.The reference antibodies was prepared by a quantitative precipitation test.In order to insure the stability of the assay,all its conditions were explored and selected.Its specificity was good and sensitivity for detecting specific IgG,IgA and IgM was 25ng/ml,150ng/ml and 220ng/ml,respectively.Its precision(error within assay):c.v.=5.4%,andreproducibility(error between assay):c.v.=13.0%.The results indicated that this assaymight be better than other assays used presently for it could measure antibodies quan-titatively and had a better precision and reproducibility.