The Expression of P53 and Phosphorylation of H2AX in Germ Cells of Varicocele Rats / 대한비뇨기과학회지
Korean Journal of Urology
; : 1354-1360, 2006.
Article
in Korean
| WPRIM (Western Pacific)
| ID: wpr-53567
Responsible library:
WPRO
ABSTRACT
PURPOSE:
To explore the expressions of P53 and phosphorylation-H2AX in varicocele-induced rat testes. MATERIALS ANDMETHODS:
A total of 16 adult male Sprague-Dawley rats underwent an operation; 12 underwent an experimental varicocele and 4, as controls, were sham-operated. Groups of 4 varicocele-induced rats underwent a left orchiectomy after 2 or 3 weeks, or both orchiectomies after 4 weeks. The sham-operated rats underwent both orchiectomies after 4 weeks. Sections of both testes from each animal were studied. The changes in the expressions of P53 and phosphorylation of H2AX were determined using immunohistochemistry and western blot.RESULTS:
Immunohistochemical staining of the left testes in the varicocele- induced rats showed that the expressions of P53 and phosphorylation of H2AX had not begun 2 weeks postoperatively, but remarkable results were observed after 3 and 4 weeks. Both testes of the varicocele-induced rats showed the expressions of P53 and phosphorylation of H2AX after 4 weeks, with the left testes being more distinctive in immunohistochemical staining compared to the right. Western blot of the left testes in the varicocele- induced rats also showed unclear expressions of P53 and gamma-H2AX after 2 weeks. Considerable distinction was seen after 3 and 4 weeks compared to the control group.CONCLUSIONS:
Our results suggest that experimental varicocele is associated with increased sperm DNA damage. These changes may be related to abnormal spermatogenesis.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Phosphorylation
/
Spermatogenesis
/
Spermatozoa
/
Testis
/
Varicocele
/
DNA Damage
/
Immunohistochemistry
/
Orchiectomy
/
Blotting, Western
/
Rats, Sprague-Dawley
Limits:
Animals
/
Humans
/
Male
Language:
Korean
Journal:
Korean Journal of Urology
Year:
2006
Document type:
Article