The regulation of the expression of membrane type-1 matrix metalloproteinase and gelatinase-A activation by progesterone in human osteoblast-like cells cultures / 中华老年医学杂志

ABSTRACT

Objective To characterize the effects of progesterone(P) on the expression of membrane type 1 matrix metalloproteinase (MT1 MMP) and gelatinase A activation in human osteoblast like cell strain MG 63 in order to understand the mechanism of progestin acting on the bone. Methods Semi quantitative RT PCR, Western blot, and confocal immunohistochemistry were used to study the actions of P on the expression of mRNA and protein of the MT1 MMP in cultured MG 63 cells. Zymogram of the gelatin and ELISA were performed to examine the effects of P on the activation of gelatinase A. Results Treatment with increasing dose of P in MG 63 cells caused a dose dependent increase in the expression of MT1 MMP mRNA ( P 0 05). Conclusions Our studies suggested that P might promote the bone formation by increasing MT1 MMP production in osteoblastic cells and thus played an important role in maintaining the osteogenic activity of osteoblasts and preparing the bone matrix for the subsequent bone cell migration and the deposition of new matrix.