Expression of human mannose-binding lectin in mice liver / 中国免疫学杂志
Chinese Journal of Immunology
; (12)1999.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-537496
Responsible library:
WPRO
ABSTRACT
Objective:
To amplify and clone the human mannose-binding lectin(MBL) gene and to express its recombinant protein in mouse liver tissue.Methods:
After the human MBL cDNA was amplified by PCR and identified by sequencing and restriction mapping,it was inserted into eukaryotic expression vector pcDNA3.The recombinant plasmid pcDNA3-MBL was injected into mice in large quantity and large volume in a short time through tail vein.These mice were sacrificed 8 h after the injection.MBL were examined in serum and hepatic tissue with Western blot and immunohistochemistry.Results:
After the human MBL gene was amplified and sequenced correctly,it was successfully inserted in the eukaryotic expression vector pcDNA3.Eight hours after the pcDNA3-MBL plasmid were injected into mice through tail veins,human MBL could be found both in serum and hepatic tissue with Western blot and immunohistochemistry examination.Conclusion:
Systemic injection of pcDNA-MBL can result in human MBL protein expression in mice liver and secretion into blood.This result may provide a new idea to treat the congenital MBL insufficient patients,who have the predisposition of infectious diseases.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
1999
Document type:
Article