Effects of tumor necrosis factor alpha(TNF-?) on the fungicidal activity of mouse peritoneal exudate cells / 中国免疫学杂志
Chinese Journal of Immunology
; (12)1985.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-537983
Responsible library:
WPRO
ABSTRACT
Objective:
To study the effect of TNF-? on the activation and fungicidal activity of mouse peritoneal exudate cells.Methods:
The mouse peritoneal exudate cells and macrophage were stimulated with IL-12、IL-18、IFN-?、TNF-? or monoclonal antibody.Cytokines and nitric oxide(NO) levels in the medium supernatant were determined with ELISA and Griess method respectively.Detected the numbers of viable Cryptococcus neoformans in macrophage.Results:
Combined with IL-12 and IL-18 synergistically induced the production of TNF-? by peritoneal exudate cells and this effect can be inhibited by neutralizing anti-IFN-? mAb.Combined with IFN-? and TNF-? synergistically induced the production of NO by macrophage and potentiate its activity against Cryptococcus neoformans.Conclusion:
The result suggests that the TNF-? plays an important role in enhancement of the fungicidal activity of macrophages. [
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
1985
Document type:
Article