Obtained DC from PBMC recovered from one time used no leukocytes transfusion apparatus / 中国免疫学杂志
Chinese Journal of Immunology
; (12)2001.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-538607
Responsible library:
WPRO
ABSTRACT
Objective:
Got high purity dendritic cells from PBMC recovered from one time used no leukocytes transfusion apparatus.Methods:
Rinsed PBMC with natural salt water from one time used no leukocytes transfusion apparatus and induced PBMC with GM-CSF and IL-4.Results:
Got 5.1?10 7 PBMC from every one time used no leukocytes transfusion apparatus and 4.7?10 6 DCs with a purity of 96.9% were obtained.These DCs express CD86(96.1%)?CD40(88.9%)?CD83(91.6%)?HLA-DR(92.9%)?HLA-ABC(99%)?CD54(97.5%) highly and a few number of these DCs could stimulate proliferation of allogeneic mixed leukocyte reactions strongly.Conclusion:
The results of this research supplied basic data for further research of DC and its clinical application.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2001
Document type:
Article