Expression of Nogo-A protein and its significance in the central nervous system of adult mice / 中华创伤杂志
Chinese Journal of Trauma
; (12)2003.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-538693
Responsible library:
WPRO
ABSTRACT
Objective To identify the cellular distribution of a my elin-associated protein (Nogo-A) in the central nervous system (CNS) of mice a nd explore its possible inhibition on the CNS axon regeneration after spinal cor d injury. Methods Brain, spinal cord, peripheral tissues and w eight-dropping injuried spinal cord from the adult C57BL/6 mice were studied. N ogo-A protein expression was localized immunohistochemically. Chick E12DRG neur ons were cultured and growth cone collapse assessed. Results N ogo-A protein expression detected was mainly in the oligodendrocyte cell body a nd the myelinated axons surrounded by cell processes rather than in the peripher al tissues. After spinal cord injury, Nogo-A was up-regulated at a moderate de gree in the area around the lesion. Chick E12 DRG growth cone collapse rate was as high as 70%, significantly higher than that in the blank control and vector control groups with a significant difference ( P
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Trauma
Year:
2003
Document type:
Article