Development of nanometer hydroxyapatite complex materials——Cytotoxicity test of nano-hydroxyapatite by MTT-assay / 实用口腔医学杂志
Journal of Practical Stomatology
; (6)1996.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-539358
Responsible library:
WPRO
ABSTRACT
Objective:
To evaluate the cytotoxicity of nano-hydroxyapatite.Methods:
Mouse lung fibroblast L-929 and primary cultured human periodontal ligament fibroblast-like cells (PDLF) were cultured in the medium of extraction of nano-hydroxyapatite or commerce hydroxyapatite at 100%,50%,10% and 0% (control) respectively.Cell growth was tested by MTT assay and the cell relative growth rate (RGR) was calculated.The cytotoxicity was graded by generally accepted standard.Results:
RGR of both cell lines cultured in nano-hydroxyapatite extraction was higher than that in control HA. In 7-day culture the cytotoxicity grade of HA was 1 in 100% of HA extraction medium and 0 in 100% of nano-hydroxyapatite extraction,or in 50% and 10% of nano-hydroxyapatite or control HA extraction medium.Primarily cultured PDLF showed lower RGR than L-929 under the same condition.Conclusion:
Nano-HA do not have cytotoxic effect.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Practical Stomatology
Year:
1996
Document type:
Article