GCV treating acute graft-versus-host disease / 中华器官移植杂志

ABSTRACT

Objective To study the therapeutic effect of TK/GCV in the treatment of acute graft-versus-host disease in H-2 haploidentical mice induced by CD34 + cells mixed with TK + T lymphocytes transplantation.Methods CD34 + cells were separated from male parental donors,bone marrows,meanwhile T lymphocytes were gained from their spleens and transduced by retroviral vectors comprising TK gene. Both were mixed and transfused into female F1 recipient mice,CD34 + cells and TK + T lymphocytes each F1 recipient mouse received were 1?10 5 and 1?10 7,respectively. GCV was administered at the dosage of 50 mg ?kg -1 ?day -1 for 7 by intraperitoneal injections on the day 0 or 7 after transplantation.Results Acute GVHDs appeared in all mice. Survival time of the mice treated with GCV on the day 0 or 7 after transplantation was ( 26.6 ? 4.8 ) days and ( 40.5 ? 8.4 ) days,respectively. Therapeutic effect of GCV on the day 7 posttransplantation was better than that on the day 0 posttransplantation ( P