Construction of recombinant nonstructural P_2C plasmid in coxsackie B4 virus / 中国免疫学杂志
Chinese Journal of Immunology
; (12)2000.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-541584
Responsible library:
WPRO
ABSTRACT
Objective:
To construct the recombinant nonstructural P_2C plasmid in coxsackie B4 virus.Methods:
Using the RT-PCR,we fishing the nonstructural protein P_2C cDNA fraction from Coxsackie B4 virus(CVB4) and cloning into the PUCm-T vector, then transfer it into E.coli to construct the recombinant plasmid.Results:
Using the total RNA of Coxsackie B4 virus as model, we amplied 987 bp fraction and cloned into PUCm-T vector identifying with BamH Ⅰ and Hind Ⅲ double enzyme.Its products were consistent with nonstructural protein P_2C PCR productions.Conclusion:
The recombinant nonstructural P_2C plasmid was constructed succesfully in coxsackie B4 virus.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2000
Document type:
Article