Effect of Intracellular Glutathione in Relieving the Cytotoxicity Induced by Arsenic / 环境与健康杂志

ABSTRACT

Objective To study the effect of cellular glutathione (GSH) in relieving the cytotoxicity induced by arsenic on HaCaT. Methods The HaCaT cells were pre-treated with N-acetylcysteine (NAC) and L-buthionine-[S'R]-sulfoximine (BSO) for 24 h, then treated with sodium arsenite (NaAsO2) by adding it into the medium for 24 h. Alarmarblue assay was used to detect the proliferation of the cells. Results The reduction rate of Alarmarblue increased when the HaCaT cells were treated with 0.001-10.000 ?mol/L of NaAsO2 alone (P