Cigarette Smoke-Induced DNA Damage and Repair in Two Kinds of Human Lung Interstitial Cells / 环境与健康杂志

ABSTRACT

Objective To study the DNA damage and repair of normal lung interstitial cells and human lung adenocarcinoma cells exposed to cigarette smoke. Methods Cultured human embryo lung fibroblasts (HLF) and human lung adenocarcinoma A549 cells. Mainstream smoke was collected by using dimerhyl sulfoxide (DMSO) and phosphate buffer solution (PBS) as absorbents. MTT assay was used to test the cytotoxicity of the solutions of cigarette smoke, then selected the concentrations of the solutions with no obvious cytotoxicity to treat cells and detected DNA damage and repair by comet assay. Results As treated with original solutions or 1/2 dilution of DMSO cigarette smoke solutions only, the Viability of cells was below 80%, but it was beyond 80% when treated with PBS solutions. The results showed that a significant difference of DNA damage was seen between the treated groups and negative control groups (P0.05),but the DNA damage caused by DMSO solutions was worse than PBS solutions significantly (P