Reconstruct the bone defects of femoral head with structural oriented biphasic calcium phosphate scaffolds based tissue engineered bone / 中华骨科杂志

ABSTRACT

Objective To observe the effect of based biomimetic biphasic calcium phosphate(BCP)tissue engineered bone masses to repair the bone defects and prevent the collapse of femoral head in dogs.Methods Biomimetic BCP ceramic scaffolds was fabricated using three-dimensional(3D)gel-lamination technology according the micro-computed tomography(Micro-CT)information of cancellous bone of dogs' femoral heads.Tissue engineered bone masses were constructed by the combination of biomimetic BCP scaffolds and induced osteogenic bone marrow stromal cells(BMSCs)in vitro.In 10 dogs,tissue engineered bone was implanted into the bone defect on the weight-bearing area of femoral head.In the control group of another 10 dogs,autogenous cancellous bone chips were impressed into the bone defect on the weight-bearing area of femoral head.The gross appearance and regeneration of new bone were observed in 30 weeks postoperatively.Results The fabricated biomimetic BCP scaffolds had better 3D structure.The trabecular system of the scaffolds had some orientation along the weight-bearing line of femoral head and represented plate-like model.BMSCs grow flourishing on the surface of the scaffolds.After 30 weeks operatively,in the tissue engineered bone implanted dogs,the gross appearances of the femoral head were integrity and a great lot of new bone tissue wraps the trabecula of the scaffolds under microscope.In the control group,the mostly femoral heads were collapse gross appearance.Conclusion Tissue engineered biomimetic BCP had better biocompatibility and could prevent the collapse of femoral head after it is implanted into the bone defects of femoral head.