Analysis of the expression of MICA on tumor cell lines of different histotypes and their sensitivity to NK cytotoxicity / 中国免疫学杂志

ABSTRACT

Objective:To investigate the expression of MHC class Ⅰ chain-related gene A(MICA) on different human tumor cells(K562,MCF-7,HR-8348,CNE-2,HeLa) and their sensitivity to NK cytotoxicity.Methods:Cytotoxicity of NK cells(isolated from 3 healthy persons) were detected by LDH releasing assay at different effect-to-target cell ratios(E∶T). The expression of MICA was measured by FACS.Results:Different level of MICA was detected on surface of tumor cell lines, anti-MICAmAb could partially inhibit the cytotoxicity of NK cells.Conclusion:NKG2D-MICA interaction can trigger the cytotoxicity of NK cells against tumor,NK cells can be a kind of effector cell for the treatment of some certain tumors.