Effect of VEGF on chondrogenic differenciation of human bone marrow derived mesenchymal stem cells / 中国矫形外科杂志

ABSTRACT

[Objective] To study the effect of vascular endothelial growth factor(VEGF)on proliferation of human bone marrow derived mesenchymal stem cells(h-MSCs)and its chondrogenic differenciation and to provide a basis for the tissue engineering technology of articular cartilage.[Method]The hMSCs were cultured in vitro as usual and its passage was induced the by medium containing 800 ng/ml or 1600 ng/ml the VEGF MSCs the cultured in the medium containing 10 ng/ml the TGF ?-1 which the had multiple the biological efficacies the to enhance the proliferation the of MSCs the and to the inhibit the activities of the inflammatory factors,acted as a positive control.The induced MSCs were used to observe its proliferation and differenciation.The synthesis of chondrocyte extracellular matrix in different induce-factor was measured by immunohisto-chemical staining.[Result]MSCs cultured wit different induced factors proliferated well,MSCs induced by VEGF medium showed a well growth activity and chondrogenic differenciation,but MSCs cultured in TGF?1-containing medium induced more chondrocyte matrix than that in VEGF medium.[Conclusion]VEGF induces chondrogenic differentiation of MSCs in vitro,but MSCs cultured in TGF?1 medium induces more chondrocyte matrix.It is suggested that VEGF acts as donor of inducer message cartilage repair by MSCs.