Effects of IL-12p35 small interference RNA on immune function of dendritic cells in rats / 中国免疫学杂志
Chinese Journal of Immunology
; (12)2001.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-545581
Responsible library:
WPRO
ABSTRACT
Objective:
To investigate the effects of IL-12p35 small interference RNA on immune functions of Dendritic cells in rats.Methods:
DCs were generated by culturing bone marrow progenitor cells of rats with GM-CSF, IL-4 and LPS in vitro. The IL-12p35 siRNA was synthesized and transfected into DCs. The expressions of CD80 and MHCⅡwere examined by flow cytometry. Reverse transcriptase PCR analysis was used to detect IL-12p35 mRNA transcription and ELISA was used to detect IL-12 and IL-10 protein levels, respectively. The antigen presenting by DCs was evaluated by mixed lymphocyte responses.Results:
IL-12p35 siRNA silenced DCs expressed lower IL-12, higher IL-10,and exhibited weak activity in stimulating the proliferation of allogenic T cells, but no changes on CD80 and MHCⅡexpressions.Conclusion:
IL-12p35 siRNA interference exerts no effects on maturation of DC, but negative effect on immume function of DCs.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2001
Document type:
Article