Role of mesenchymal stem cells in rat allogeneic bone marrow transplantation / 中国免疫学杂志

ABSTRACT

Objective:The effect of rat mesenchymal stem cells(MSCs)on graft versus host disease after allogeneic co-transplantation with bone marrow in rat was investigated.Methods:MSCs from bone marrow cells of rat were isolated and cultured in vitro.The purity of MSCs was identified with the spindle-fibroblastic morphological characterization and the phenotypes were analyzed with flow cytometry.The suppressive effect of MSCs on T cell proliferation was analyzed using mixed lymphocyte cultures(MLR).Allogeneic BMT model from SD rats to Wistar rats was established,and then,MSCs were co-transplanted with bone marrow cells at different ratio,the suppressive effect of MSCs on GVHD was studied by clinical symptom,Kaplan-Meier survival curve,and histological analysis.Results:In vitro,when MSC were added to the mixed lymphocyte cultures,the proliferation of T cells were inhibited markedly in a dose-dependent manner(P