Biological differences between healing and normal medial collateral ligament fibroblasts / 中国矫形外科杂志
Orthopedic Journal of China
; (24)2006.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-546340
Responsible library:
WPRO
ABSTRACT
[Objective]To examine the difference of proliferation and ?-smooth muscle actin(?-SMA) expression between healing and normal medial collateral ligament(MCL) fibroblasts.[Method]Ten adult,male,Sprague-Dawley rats weighing between 350 and 375 g were used in this study.Normal and healing MCL were cut into small pieces in aseptic conditions,and then placed and cultured in culture chamber.Fibroblasts were passaged with 0.25% trypsin.After 24 and 48 hours incubation,MTT was used to measure the cell proliferation.The production of ?-SMA was measured by Western Blot.[Result]After 24 and 48 hours incubation,healing fib roblasts showed absorbency values of 0.49?0.080 and 0.53?0.07 respectively.II was found that healing fibroblasts proliferated faster than normal fibroblasts(P
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Orthopedic Journal of China
Year:
2006
Document type:
Article