Effect of ING4 gene transfection on biological characteristics of human tumor cell line SGC-7901 / 中国免疫学杂志

ABSTRACT

Objective:To investigate the effect of IGN4 gene trasfection on SGC-7901 cells,and the possible mechanism for its anti-tumor effect .Methods:Ad-ING4 was obtained by gene recombination and packaging technique in vitro.The expression of ING4 mRNA and protein were analyzed by RT-PCR and western-blot respectively.The effect of Ad-ING4 on growth of SGC-7901 cells was detected by MTT.Apoptotic cells were detected by Laser Scan Co-focal Microscope (LSCM).The expression of p21 in SGC-7901 cells was analyzed by western-blot.Results:After infection with Ad-ING4,the expression of ING4 mRNA and protein were showed in SGC-7901 cells detected.The proliferation of SGC-7901 cells was inhibited significantly(P