Effect of L-glutamine on liver Bcl-2 mRNA expression after total hepatic inflow occlusion in rats / 中国现代普通外科进展
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-546608
Responsible library:
WPRO
ABSTRACT
Objective:
To explore the effect of L-glutamine(Gln) on liver Bcl-2mRNA expres sion and apoptosis after total hepatic inflow occlusion in rats.Methods:
Male Wistar rats were assigned randomly to three groups(n=40)Group A sham-operation group,group B control group,rats were pretreated with 4 ml 0.9% saline intraperitonally twice per day on 5 consecutive days,group C,rats were pretreated with Gln dissolved in 4 ml 0.9% saline intraperitoneally twice per day on 5 consecutive days.The rats from group B and C underwent total hepatic inflow occlusion for 35min by the pringle's manoeuvre.Ten rats from each group were randomly chosen and killed before the initiation of occlusion at 2 h,4 h,24 h after reperfusion respectively.The levels of MDA,GSH in liver tissue were measured.The serum concentrations of ALT,AST,LDH were assayed on a standard biochemistry autoanalyser.The expression levels of Bcl-2 mRNA in liver were assessed by RT-PCR.The apoptosis of liver were observed by DUTP method.The percentage of apoptosis was analyzed.Results:
Compared with group B,the levels of GSH in group C increased after reperfusion(P
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Current Advances in General Surgery
Year:
1999
Document type:
Article