Determination of Insulin Receptors of Erythrocytes of Four Different Kinds of Animals / 第三军医大学学报

ABSTRACT

This article is to introduce our method to determine the erythrocytic insulin receptors of four different species of animals including human being. A small amount of blood with Normal buffer as incubation medium was put at 4℃ over night and then the binding rate of the erythrocytes with 125I-insulin was tested. The highest binding rates (B/T%)of the erythrocytes from human beings, rabbits, dogs and rats were within the range from 10%to15% and the average of nonspecific binding rates was 3.5%. In different animals, the number of erythrocytic insulin receptors was also different, which was the largest in dogs, then in rabbits and in rats, and the smallest in human beings. The competition inhibition curve of the binding of the erythrocytic insulin receptors with 125I-insulin accorded with the nonlinear Scatchard plots and was similar to the results reported by other authors.Our experiment showed that good results could also be obtained if Normal Buffer was used ot subsitute Buffer G as the incubation medium. Moreover, the degradation rate of insulin-receptor Complex could be reduced when the test was carried out at 4℃,so this method would be useful to assay the erythrocytic insulin receptors in clinical practice.