Experimental study Detecting Entroviruses RNA With the Method of cDNA: RNA Spot Hybridization / 第二军医大学学报
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-550631
Responsible library:
WPRO
ABSTRACT
Taking Coxsackie B3 virus cDNA labelled by Biotin as detecting probes and 25 kinds of standard enteroviruses as detected viusr, a spot nucleic acid hybridization method has been established to detect enterovirus RNA gene. The method has simple operation and its sensitivity is 50-80 TCID50 spot. Reagent is safe and stable, and may be stored for long period. pCBHⅢ/35.51 probe used has wider hibridization spectrum to enteroviruses; pCBⅢ/29 only reacts with RNA of Coxsackie B3 virus in strict condition. Consequently, the hybridization character which these probes possess is suitable to determining enlerovirus in clinical samples.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
1985
Document type:
Article