Effect of antisense phosphorothioate oligonucleotide on activity of telomerase for human bladder tumor cell / 临床外科杂志
Journal of Clinical Surgery
; (12)2002.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-553401
Responsible library:
WPRO
ABSTRACT
Objective To detect the Telomerase inhibitor-antisense phosphorothiate oligonucleotide (asONS) effect on telomerase activity of human bladder tumor cell line BIU-87.Methods BIU-87 cells and cell extracts are cultured with asONS,non-asONS and control fluid at different concentrations and times respectively.Telomerase activity was assayed by TRAP PCR-ELASA.Results AsONS can inhibit the activity of BIU-87 cells ,whereas non-asONS and control group not, and have significant difference(P
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Clinical Surgery
Year:
2002
Document type:
Article