Oridonin induced HeLa cell apoptosis through capase pathway / 中国药理学通报

ABSTRACT

AIM To study the mechanisms of oridonin-induced HeLa cell apoptosis. METHODS Morphological observation, agarose gel electrophoresis, LDH release and caspase inhibitors were used. RESULTS Oridonin had significant apoptotic effect on HeLa cells in a dose- and time-dependent manner. The marked morphological changes including condensed chromatin, nuclear fragmentation and apoptotic bodies and DNA ladder in agarose gel was observed. The activity of caspase-3 was increased about 2 times of control value after treatment with oridonin. CONCLUSION Oridonin induces HeLa cell apoptosis through caspase pathway.