EFFECT OF pH ON THE MUTAGENIC POTENCY OF ICR-170 IN THE REPRODUCTIVE GLANDS OF FEMALE SCHISTOSOMA JAPONICUM / 中国血吸虫病防治杂志

ABSTRACT

Objective To explore the effect of pH on the mutagenic potency of ICR-170 in the reproductive gland of female Schistosoma japonicum.Methods Mice of Kunming strain were infected with S.japonicum cercariae which were previously treated with acridine mutagen ICP-170 at various pH values(pH7.2-8.0) for 30 min or 45 min. Results The pH of the solution had significant effects on the mutagenic potency of ICR-170 in female S.japonicum. When the pH value increased from pH 7.2 to pH 8.0,the percent of mutation of female reproductive organs decreased. The highest mutagenic rate of ICR-170 occurred at pH 7.2,which was 13 times of that at pH 7.8 in the group of 10 ?g/ml 45 min,6 times in the group of 15 ?g/ml 30 min. However,pH value had lower effect on recovery of adult warms compared with mutagenicity. Conclusion The mutagenic potency of ICR-170 is strongly pH-dependent in the reproductive glands of female S.japonicum.