Apoptin induces G_2-M arrest in cancer cells / 北京大学学报(医学版)
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-555083
Responsible library:
WPRO
ABSTRACT
Objective:
To further study the effect of apoptin in inducing cancer cell specific apoptosis and the possible applications in cancer therapy.Methods:
Apoptin gene was amplified by PCR and inserted into pcDNA3.1(+) with a FLAG tag in front of the multi-cloning-site. Apoptin gene with the FLAG tag was sub-cloned into an adenovirus vector. Several cancer cell lines were transfected with pcDNA3. 1/FLAG/apoptin or infected with apoptin containing recombinant adenoviruses to study the morphologic changes. Ad/apoptin infected cells were also analyzed by flowcytometry after staining with PI.Results:
Expressed apoptin was localized in the nucleus of cancer cells. Chromatin condensation occurred 2 or 3 days after Ad/apoptin(+) infection. Cell number in G 2-M phase increased dramatically after Ad/apoptin(+) infection.Conclusion:
Apoptin can induce cell cycle G 2-M arrest and chromatin condensation in cancer cells.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Peking University(Health Sciences)
Year:
2004
Document type:
Article