Effects of berberine on potassium current of guinea pig colonic smooth muscle cells / 中国药理学通报

ABSTRACT

AIM To clarify the mechanism of berberine on mobility diarrhea by investigating the effects of berberine on calcium-activated potassium current I K(Ca) and delayed-rectifier potassium channel current I K(V) of guinea pig colonic smooth muscle cells. METHODS Single guinea pig colonic smooth muscle cells was isolated by collagenase; The effects of berberine on I K(Ca) and I K(V) were detected by using patch clamp technique, under the conventional whole cell patch clamp mode. RESULTS 10, 50, 100 ?mol?L -1 berberine inhibited I K(Ca) of guinea pig colonic smooth muscle cells significantly (P