Effects of hydrogen peroxide on intracellular free calcium concentration in cultured neonatal rat cardiac myocytes and its antagonism by carvedilol / 解放军医学杂志
Article
in Zh
| WPRIM
| ID: wpr-555190
Responsible library:
WPRO
ABSTRACT
Objective To examine effects of hydrogen peroxide on intracellular free calcium concentration(i) in cardiac myocytes and its antagonism by carvedilol. Methods A cell culture of neonatal rat cardiac myocytes was used for experimentation. They were divided into four groups, i.e. control group, hydrogen peroxide (H 2O 2) group, carvedilol group,and H 2O 2 + carvedilol group. Confocal microscope was used with Fluo-3/AM as calcium indicator to detect changes in i immediately and 15 minutes after H 2O 2 intervention, respectively. Results The intracellular fluoresence intensity of a single cardiae myocyts in the control group and carvedilol group was low. The intracellular fluoresence intensity of a single cardiac myocyte in H 2O 2 group was significantly higher than in the control group 15 minutes after intervention (P
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Medical Journal of Chinese People's Liberation Army
Year:
2001
Document type:
Article