Role of P38 mitogen-activated protein kinase in expressing of cyclooxygenase-2 in human umbilical vein endothelial cells / 医学研究生学报

ABSTRACT

Objective: To investigate the role of P38 mitogen-activated protein kinase (P38MAPK) in expressing cyclooxygenase-2 (COX-2) in human umbilical vein endothelial cells (HUVEC), in order to study the role of P38MAPK on diabetic atherosclerosis. Methods: HUVEC are separately stimulated with high glucose, advanced glycosylation end products (AGE), high insulin and H_(2)O_(2), the expression of phospho-P38MAPK and COX-2 is detected; Simultaneously, HUVEC pre-treated with SB203580 (P38MAPK special inhibitor) are stimulated with above stimulating factors, the expression of COX-2 is detected. Results: High glucose, AGE, high insulin and H_(2)O_(2 ) can activate P38MAPK and increase the expression both of phospho-P38MAPK and COX-2 significantly; But the expression of COX-2 is inhibited by SB203580. Conclusion: P38MAPK regulates the expression of COX-2; P38MAPK is a upstream signal molecule of COX-2 and P38MAPK may be one of initial signals on atherosclerosis occurrence.