Influence of tretinoin on neural specific gene expression in fetal liver cells and its signaling pathways / 第二军医大学学报
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-555362
Responsible library:
WPRO
ABSTRACT
Objective:
To study the mechanism of tretinoin-induced neural differentiation in fetal liver CD34+ cells in vitro.Methods:
CD34+ cells from fetal liver were isolated with a magnetic cell sorting kit and were cultured. Cells pretreated with or without protein kinase C(PKC) inhibitor chelerythrine chloride(3 ?mol/L) were induced by 5?10-7mol/L tretinoin for 24 h, and then incubated in serum-free medium. Expressions of genes in treated cells were assayed by Western blotting and RT-PCR.Results:
Tretinoin significantly promoted expression of neural specific genes such as Nestin, neuron-specific nuclear protein (NeuN) , neuron-specific neuronfilament-M (NF-M) , and MAP-2 in fetal liver CD34+ cells, with Nestin increased by 4. 09 folds, NeuN by 5. 12 folds, and NF-M by 7. 27 folds (all P
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2000
Document type:
Article