Mitochondrial membrane potential change and apoptosis of small intestinal smooth muscle cells in diabetic rats / 解放军医学杂志

ABSTRACT

Objective To investigate the relationship between mitochondrial membrane potential change and apoptosis of smooth muscle cells of the small intestine in diabetic rats. Methods Seventy Wistar rats were randomly divided into two groups diabetic group(STZ60mg/kg intraperitonealy) and control group.The gastric empty time and intestinal transit were determined in diabetic rats 2 months after the reproduction of diabetes. Mitochondrial membrane potential in small intestinal smooth muscle cells was determined by the change in the intensity of labeling rhodamine 123 with lasor scanning confocal micrographs, and apoptosis index was assessed with the technique of terminal deoxynucleotidyl transferase mediated d-UTP nick end labeling(TUNEL) test and flow cytometry.The changes in expression of cytochrome C were determined by Western blotting. Results The mitochondrial membrane potential of small intestine smooth musele cells was significantly lowered in diabetic rats compared with normal rats. Apoptosis index in diabetic rats was significantly higher than that of normal as shown by TUNEL technic. Apoptosis rate in diabetic rats was 15%, and it was significantly higher than that of normal rat (P