Influence of thrombospondin 1 on the expression of VEGF in human renal tubular epithelial cells / 解放军医学杂志

ABSTRACT

Objective To investigate the influence of thrombospondin 1 (TSP-1) on the expression of vascular endothelial growth factor (VEGF) in human renal tubular epithelial cells. Methods Human renal tubular epithelial cell (HKCs) line was cultured in vitro with DMEM/F12 1∶1 medium. HKCs were divided into four groups as follows normal control group [HKCs were cultured with serum free medium (FSM), C group], TGF-? 1 group (HKCs were treated with FSM containing TGF-? 1, T group), antisense oligonucleotide group [HKCs were treated with FSM contained TGF-? 1 and TSP-1 antisense oligonucleotide (AS), TA group], missense oligonucleotide groups [HKCs were treated with FSM contained TGF-? 1 and TSP-1 missense oligonucleotide (SC), TS group]. Cells were cultured 72 hours in the conditioned media. The expressions of TSP-1 and VEGF were detected with immunohistochemistry. The expressions of TSP-1 mRNA and VEGF mRNA were assayed by RT-PCR. The correlation between the relative expressions of VEGF mRNA and TSP-1 mRNA was analyzed. Results There were significantly increased expressions of VEGF and VEGF mRNA in TA groups (P0.05) compared with T groups. The expressions of VEGF and VEGF mRNA were decreased significantly in T groups and TS groups (P0.05) compared with T groups. The expressions of TSP-1 and TSP-1 mRNA were increased significantly in T groups and TS groups (P