Detection of the creatine kinase activity and CK isozyme activity of black bear / 第三军医大学学报

ABSTRACT

Objective To detect the creatin kinase (CK) and its isozeyme (CK-MB) activity in serum of the healthy black bear (Ursus thibetanus) reared in pens to establish reference standard of biochemistry for CK and CK-MB activities of black bears as well as to offer scientific basis for prevention and cure of the black bear disease. Methods The CK activity and CK-MB activity were measured using spectrophotometer based on the principle of enzymatic reaction rate. All data were used to calculate the mean value, variation coefficient and the confidential interval of expected value of enzymatic activity. Results Under 30 ℃, of the male black bears, the average CK activity is 162.70 U/L, the variation coefficient 11. 90%, the confidential interval of expected value 148.90 to 176.50 U/L, and the average CK-MB activity is 17.4 U/L, the variation coefficient 17%, the confidential interval of expected value 15.26 to 19.54 U/L. Of the female black bears, the average CK activity is 149.3 U/L, the variation coefficient 8%, the confidential interval of expected value 140.68 to 157. 92 U/L, and the average CK-MB active is 15.8 U/L, the variation coefficient 18.6%, the confidential interval of expected value 13.70 to 17.90 U/L. Conclusion The data we obtained can serve as biochemical indexes for the healthy black bears.