Subtance P expression in mandibular callus after section / 第三军医大学学报

ABSTRACT

Objective To study the effect of sensory nerve on mandibular fracture through detecting the expression of SP in mandibular callus after inferior alveolar nerve (IAN)section. Methods Thirty-two Chinese rabbits were divided into two groups. All rabbits suffered from a standardized fracture in the left mandibula, and half of them underwent IAN section. The callus were stained by hematoxylin and immunochemistry to observe the expression of SP in callus and fracture healing process. Results There were a larger quantity of fibrous callus formation, relatively immatured woven bone callus, lower proportion between immatured bone callus and new bone callus and less immunoreactive substance P in the group with IAN section than in the group with intact IAN at day 7, 14, 21, 28 after operation. Strong immunoreaction of substance P occurred mainly in bone marrow on day 28 postoperatively in both groups. Conclusion Sensory nerve may involve in the process of callus formation at early stage and bone remodeling at late stage so that sensory nerve injury influences the quantity and quality of new callus formation. It seems that neural involvement in bone healing is related to the local concentration of SP in the callus.