Expression change of tissue factor in rat carotis endothelial cells induced by shear stress in vivo / 第三军医大学学报

ABSTRACT

Objective To investigate the expression of tissue factor(TF) in endothelial cells induced by fluid shear stress and the mechanism of the TF expression in vivo.Methods Fifty-four male SD rats weighing 300?15.5 g were randomized to 9 groups(n=6 in each group) one as control and another eight groups were assigned into corresponding time points of 0.5,1,3,6,12 h and 1,3,7 d after stenosis establishment of the left arteria carotis.The mRNA and protein expression of TF factors,nuclear factor Sp-1 and Egr-1 were examined in left arteria carotis communis intima by using in situ hybridization and immunohistochemistry.The results were processed by computer image analysis.Results The mRNA and potein of TF,Egr-1 and Sp-1 were faintly expressed in intima of control group.After exposed to stenosis for 30 min,the staining intensity was increased.The mRNA and protein expresson of TF markedly increased as compared with control group((P