Time and distribution characteristics of neuron apoptosis in rat after ischemic insult / 第三军医大学学报

ABSTRACT

Objective To investigate the time and distribution characteristics of neuron apoptosis occurred after global cerebral ischemia in rats,so as to provide some helpful experimental basis both for the early interference of injured neuron and the prevention and treatment of brain secondary damage.Methods In the global cerebral ischemia model with modified 4-vessel occlusion method,the amount and distribution of apoptotic neuron in hippocampus and cortex after ischemia and reperfusion were detected with TTC staining,TUNEL labeling and AO/EB fluorescence examination techniques.Intracellular free calcium concentration was measured with Fura-2 AM fluorescent indicator.Results TUNEL showed that apoptotic neurons scattered in hippocampus 3 h after reperfusion,then spread around to cortex.The amount of apoptotic cell reached to the peak at 24-48 h.The died cells appeared later and mainly dispersed around the apoptotic cells.There was a significant increase of the amount of died cells 72 h after ischemia and reperfusion.AO/EB fluorescence examination showed that the total amount of the apoptotic neurons in hippocampus,frontal cortex and parietal cortex reached their peak at 24 h and 72 h respectively,and they were significantly higher than that in control group(P