Studies on ?-cobro-neurotoxin-loaded Poly(lactic-co-glycolic Acid) Nanoparticles: Preparation and Pharmacokinetics in vivo through Nasal Administration / 浙江中医药大学学报

ABSTRACT

[Objective] To investigate the possibility of [poly(lactic-co-glycolic acid) ,PLGA] nanoparticles (NP) as a new kind of protein drug carrier for nasal administration. [Method] CNT-PLGA-NP was prepared by double emulsification solvent evaporation. Its morphology was examined by transmission electronmicroscope (TEM). In the use of photon correlation spectroscopy(PCS) and laser Doppler anemometry(LDA),its Zeta potential and mean particle size were estimated .Also, the entrapment efficiency of CNT-PLGA-NP and its pharmacokinetics in rats through nasal administration were studied. [Result] The Zeta potential, mean particle size, entrapment efficiency of CNT-PLGA-NP were-13.4mV, 320.20nm and 45% respectively .Its vitro CNT release from CNT-PLGA-NP appeared to consisting of two components with initial rapid release followed by a exponential stage.The AUC and t1/2(?) of CNT, CNT-PLGA-Np and CNT-PLGA-NP(B+T)were 1.14, 8.37, 7.12?g?h/mL and 20.06, 44.14, 34.82h respectively. [Conclusion]PLGA-NP might be a potential new drug carrier for CNT.Polysorbate 80 and borneol might be good absorption enhancers.