Rat chronic gastroesophageal reflux disease model: a study of 3 preparation methods / 第二军医大学学报

ABSTRACT

Objective:To introduce 3 methods for preparation of stomach-conserving gastroesophageal reflux disease(GERD)model in rats,so as to improve the quality and successful rate of model preparation.Methods: Male Sprague-Dawley rats were divided into sham-operated(SO) group(n=20) and 3 stomach-conserving reflex model groups duodenogastroesophageal reflux(DGER)group(n=47,esophagogastroduodenal anastomosis),gastroesophageal reflux(GER)group(n=20,partial pyloric ligation + cardiomyotomy followed by gastric fundus-esophagus anastomosis) and duodenoesophageal reflux(DER) group(n=21,jejuno-esophageal anastomosis followed by duodeno-esophageal anastomosis).Rats were observed closely after operation and their body weights were monitored.The rats that died unexpectedly were autopsied immediately and the surviving rats were sacrificed to observe the esophageal pathological changes at 1,2,3,and 4 months after operation.Results: Three rats died due to anesthetic accidents;26(24.1%) died unexpectedly after operation,18(38.3%)in DGER group,1(5%) in GER group,and 7(33.3%)in DER group;no rat died in SO group.Necropsy showed that the death cause of rats included obstruction,infection,asphyxia,hemorrhage,anastomosis leakage and perforation,etc.Esophageal pathological changes were noticed in all rats of 3 model groups;the life quality of surviving rats was good.Conclusion: The 3 methods for establishing rat stomach-conserving GERD model can produce high quality model and high rate of pathological changes,but with high post-operation mortality.In addition to rational operative methods,avoiding post-operation complications and unexpected death are also very important for successful GERD model preparation.