Study of the relationship between cardiac fibrosis and macrophages in spontaneously hypertensive rats(SHRs)-the interventional experiment of Benazepril / 中国药理学通报

ABSTRACT

Aim To explore the role of macrophages in the progression of fibrosis in spontaneously hypertensive rats and study effects and possible mechanisms of benazepril.Methods 12-week-old male SHRs were divided into hypertension group(SHR,n=10),benazepril treatment groups(BenL,BenH subgroup;Rats received benazepril 1.7 or 17 mg?kg-1?d-1 intragastric administration,respectively;each n=10),and age and sex matched Wistar Kyoto rats were set as control group(WKY,n=10).After 18 weeks' intervention,systolic blood pressure(SBP),left ventricular mass(LVM),left ventricular mass index(LVMI),contents of left ventricular collagen and macrophages were examined.Transcriptional levels of TGF-?_1 and interleukin-6(IL-6)were evaluated by reverse transcription polymerase chain reaction(RT-PCR).Results The amount of macrophages in SHR group was strikingly increased and transcriptional levels of TGF-?_1 and IL-6 in SHR group were much higher when compared with WKY group(P