Screening of brain-derived neurotrophic factor-like compounds / 中国药理学通报
Chinese Pharmacological Bulletin
; (12)2003.
Article
in Zh
| WPRIM
| ID: wpr-562018
Responsible library:
WPRO
ABSTRACT
Aim To apply simple combined models to screen small molecule BDNF-like compounds for potential pharmacotherapy of central nerve degenerative diseases.Methods BDNF-like bioactivities were dentified with effects against apoptosis induced by serum withdrawal in turn in cultured human SH-SY5y cells,rat NIH3T3/trkB cells vs NIH3T3 cells,using MTT technique.Results More than 400 compounds were tested by using these methods.11 of them presented protective effects on apoptosis induced by serum withdrawal in SH-SY5y cells.2 of them also provided selective protection against apoptosis induced in NIH3T3/trkB cells versus in NIH3T3 cells,which were identified as BDNF-like compounds.Further study showed that these 2 compounds could withstand SH-SY5y cell injuries induced by 6-OHDA.Conclusion All the above studies provide a useful series of simple models for BDNF-like drug screen and some compound candidates for further study of neuroprotection.
Full text:
1
Database:
WPRIM
Type of study:
Diagnostic_studies
/
Prognostic_studies
/
Screening_studies
Language:
Zh
Journal:
Chinese Pharmacological Bulletin
Year:
2003
Document type:
Article