The Effects of P27~(KIP-1) on Proliferation of Airway Smooth Muscle Cells in Asthmatic Rats / 医学研究杂志

ABSTRACT

Objective To investigate the effects of P27Kip-1 on proliferation of airway smooth musde and airway remodeling in asthmatic rats.Methodes 30 healthy,male SD rats were randomly and equally divided into three groupscontrol group(C),asthmatic 4 weeks group(A1),asthmatic 6 weeks group(A2).A rat asthma model was developed by the sensitized with ovalbumin and Al(OH)3,and repeatedly exposed to aerosolized ovalbumin.After the last challenge,The animals were evaluated for AHR,the airway wall thickness(Wat /Pbm)and the bronchial smooth muscle thickness(Wam/Pbm)were measured by image analysis system.The expression of P27Kip-1 and PCNA was determined by immunohis-tochemical staining.Results(1).The expression of P27Kip-1 protein in two asthmatic groups was significantly lower than that of the control group,respectively(P