Effect of new QingKaiLing injection on adhesion molecules expression of an in vitro ischemia and reperfusion injury model of rat brain microvascular endothelial cell / 中华中医药杂志
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-563096
Responsible library:
WPRO
ABSTRACT
Objective:
To observe the expression of cell adhesion molecules ICAM-1 and VCAM-1of cultured rat brain microvascular endothelial cells(MVEC),expecting to explore the mechanisms of new QingKaiLing injection protecting brain from injury of inflammatory cascade in cerebral ischemia diseases.Methods:
Rat cerebral MVEC were extracted by separating microvessel sections and collagenase enzymatic digesting,an in vitro ischemia reperfusion model was established(Kreb,95%N2+5%CO2),the protein and mRNA expression of ICAM-1 and VCAM-1 were detected by using immunocytochemical stain and RT-PCR method.Results:
The expression of adhesion molecules of model group were significantly higher than those of noral group(P
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Journal of Traditional Chinese Medicine and Pharmacy
Year:
2005
Document type:
Article