Effects of atorvastatin on myocardium and expression of NF-?B and TNF-? in a rat model of type 2 diabetes mellitus / 解放军医学杂志

ABSTRACT

Objective To investigate the effect of atorvastatin on cardiac fibrosis and on the expression of NF-?B and TNF-? in a rat model of type 2 diabetes mellitus.Methods Sixty SD rats were randomly divided into 3 groups.In control group(n=20) the rats were fed with normal food and gavaged with normal saline.In diabetes group(n=20) the rats with type 2 diabetes was induced by intraperitoneal injection of low doses of streptozotocin(STZ),and the rats were fed with high fat diet.In atorvastatin group(n=20) diabetes was reproduced in rats and atorvastatin 20mg/(kg?d) was gavaged for 12 weeks.The pathological changes in myocardial cells were observed under light microscope.Collagen content was observed by Masson staining.The contents of TNF? were determined in plasma and cardiac tissue with RIA.RT-PCR was used to study the gene expression of NF-?B and TNF-?.The proteins of NF-?B p65 and TNF-? were assayed by immunohistochemical method.Results It was showed under light microscope that cardiac myocytes were compact and orderly with clear structure in control group.The cardiac myocytes were disorderly,distorted,with infiltration of inflammatory cells in the interstitium in diabetes group.The cardiac myocytes were orderly arranged,and the structure was close to normality in atorvastatin group.TNF-? in diabetic rats increased significantly in plasma and myocardium(P