Connective tissue growth factor inhibits apoptosis of human hypertrophic scar fibroblasts through phosphatidylinositol 3-kinase/protein kinase B signal pathway / 第三军医大学学报

ABSTRACT

Objective To explore whether the anti-apoptotic effect of connective tissue growth factor on human hypertrophic scar fibroblasts is mediated by phosphatidylinositol 3-kinase/protein kinase B(PI3K/PKB)signal pathway.Methods The cultured human hypertrophic scar fibroblasts were divided into three groups:control group,connective tissue growth factor(10 ng/ml)stimulated group,the group pretreated with PI3K inhibitor LY294002(10 ?mol/L)and stimulated with connective tissue growth factor.Levels of phosphorylated PKB were examined by Western blotting in human hypertrophic scar fibroblasts that were stimulated with connective tissue growth factor for 30 min.Flow cytometry was used to detect the apoptosis ratio of the cells.Results Compared with the control group,the apoptosis ratio of the connective tissue growth factor stimulated group decreased(P