Establishment of Experimental Conditions for Pathogen Inactivation of Riboflavin Combining with Ultraviolet Light Radiation / 医学研究杂志

ABSTRACT

Objective To design experimental conditions of Riboflavin combining with ultraviolet light illumination for pathogen inactivation and to verify its effect.Methods The effects of multi-factors,such as Riboflavin concentrates,dosage of ultraviolet irradiation,pH value and medium,were observed on E.coli inactivation by photochemical method when it was used as an indicating germ in phosphorus solution(PBS)and taken count by smearing Mac-lonkey agar plates for colony-forming units.Results The reduction of E.coli reached 3.87 logs when the system was treated with 12.5 umol/L riboflavin and 3.0mJ/ml of ultraviolet light at 254nm in PBS medium.Conclusion E.coli in PBS medium was sensitized to low concentrates of Riboflavin and low dosage of ultraviolet irradiation.