Determination of Amino Acids in Silkworm Extract by High Performance Liquid Chromatography with Precolumn Derivatization / 医学研究杂志

ABSTRACT

Objective To determin the amino acids in silkworm extract by High Performance Liquid Chromatography with Precolumn Derivatization.Methods With the technology of precolumn derivatization, along with high performance liquid DABS-CL and a high-efficiency YMC-Pack ODS-A column(250?4.6mm, 5 ?m), with mobile phase being A25mol/L KH2PO4 and B acetonitrile methyl alcohol =7030, at flow rate of 1.5 ml/min, peaks were detected at 436nm and column temperature being 35℃.Results All kinds of amino acids have a nice linear relations (r=0.9989~0.9999), precision (RSD